Recombinant Rat I-PTH Protein (Trx Tag)

Specifications

Catalog number

PDER100113-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.

Abbreviation

I-PTH

Synonyms

IPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

UniProt

P04089

Accession Number

P04089

Expression System

E.coli

Tag

N-Trx

Sequence

Ala32-Gln115

Field of Research

Signal transduction

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.1 kDa

Observed Molecular Weight

32 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Rat