Recombinant Rat Cst3 Protein (Sumo Tag)

Specifications

Catalog number

PDER100117-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 120 amino acid residues, with a molecular mass of 13,343-13,359 Da, and containing four characteristic disulfide-paired cysteine residues. Cystatin C is a low-molecular-weight protein that has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. In almost all the clinical studies, Cystatin C demonstrated a better diagnostic accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. Cystatin C could be a useful clinical tool to identify HIV-infected persons. In addition, its expression is up-regulated in malignance of certain tumor progression.

Abbreviation

Cst3

Synonyms

AD 8; AD8; Amyloid angiopathy and cerebral hemorrhage; ARMD11; bA218C14.4 (cystatin C) ; bA218C14.4; Cst 3; Cst3; CST3 protein; Cystatin 3; Cystatin-3; Cystatin-C; Cystatin3; CystatinC; CYTC; Epididymis secretory protein Li 2; Gamma trace; Gamma-trace; HCCAA; HEL S 2; MGC117328; Neuroendocrine basic polypeptide; Post gamma globulin; Post-gamma-globulin

UniProt

P14841

Accession Number

P14841

Expression System

E.coli

Tag

N-Sumo

Sequence

Gly21-Ala140

Field of Research

Cancer; Cell Biology; Cardiovascular; Stem Cells; Tags and Cell Markers

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

26 kDa

Observed Molecular Weight

32 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Rat