Recombinant Rat CCL20/MIP-3α Protein (Trx Tag)

Specifications

Catalog number

PDER100136-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Chemokine (C-C motif) ligand 20 (CCL20/MIP-3α) or liver activation regulated chemokine (LARC) or Macrophage Inflammatory Protein-3 (MIP3A) is a small cytokine belonging to the CC chemokine family that attracts immature dendritic cells and memory T lymphocytes, both expressing CCR6. Depending on the cell type, this chemokine was found to be inducible by cytokines (IL-1beta) and by bacterial, viral, or plant products (including LPS, dsRNA, and PMA) . MIP3A / CCL20/MIP-3α is Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of MIP3A / CCL20/MIP-3α has been seen in thymus, prostate, testis, small intestine and colon. As a chemotactic factor, MIP3A / CCL20/MIP-3α attracts lymphocytes and, slightly, neutrophils, but not monocytes. This chemokine may Inhibit proliferation of myeloid progenitors in colony formation assays and it may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. Its C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Chemokine CCL20/MIP-3α was shown to play a role in colorectal cancer (CRC) pathogenesis.

Abbreviation

CCL20/MIP-3α

Synonyms

CCL; CKb; Scya; CKb4; LARC; MIP3A; Scya20; St38; Ccl20

UniProt

P97884

Accession Number

P97884

Expression System

E.coli

Tag

N-Trx

Sequence

Ser27-Met96

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.6 kDa

Observed Molecular Weight

31 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Rat