Recombinant Human IL5 Protein (His Tag)

Specifications

Catalog number

PDMH100203-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with an the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF) . This gene is located on chromosome 5 with anin a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

Abbreviation

IL5

Synonyms

B-cell differentiation factor I; Colony stimulating factor; EDF; Eosinophil differentiation factor; IL-5; IL5; IL5; Interleukin 5 (colony stimulating factor; eosinophil) ; Interleukin 5; Interleukin-5; T Cell Replacing Factor; T-cell replacing factor; TRF

UniProt

P05113

Accession Number

P05113

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Ser134

Field of Research

Cancer; Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

14.6 kDa

Observed Molecular Weight

15-20 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human