Recombinant Human CD30L Protein (Fc Tag)

Specifications

Catalog number

PDMH100218-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.

Abbreviation

CD30L

Synonyms

CD153; CD153 antigen; CD30 antigen ligand; CD30 L; CD30 ligand; CD30-L; CD30L; CD30LG; MGC138144; TNFL8; Tnfsf8; Tumor necrosis factor (ligand) superfamily member 8; Tumor necrosis factor ligand superfamily member 8

UniProt

P32971

Accession Number

P32971

Expression System

Mammalian

Tag

C-Fc

Sequence

Gln63-Asp234

Field of Research

Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.8 kDa

Observed Molecular Weight

50-70 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human