Recombinant Human CRTAM Protein (His Tag)

Specifications

Catalog number

PDMH100222-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The CRTAM gene is upregulated in CD4 (see MIM 186940) -positive and CD8 (see CD8A, MIM 186910) -positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains. Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo.

Abbreviation

CRTAM

Synonyms

CD355; Class I MHC restricted T cell associated molecule; Class-I MHC-restricted T-cell-associated molecule; CRTAM; CRTAM; Cytotoxic and regulatory T cell molecule; Cytotoxic and regulatory T-cell molecule

UniProt

O95727

Accession Number

O95727

Expression System

Mammalian

Tag

C-His

Sequence

Ser18-Gly287

Field of Research

Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.5 kDa

Observed Molecular Weight

55-65 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human