Recombinant Human GM-CSF Protein (Fc Tag)

Specifications

Catalog number

PDMH100230-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

Abbreviation

GM-CSF

Synonyms

Colony stimulating factor 2 (granulocyte-macrophage) ; Colony Stimulating Factor 2; Colony stimulating factor; Colony-stimulating factor; CSF 2; CSF; CSF2; CSF2; GM-CSF; GMCSF; Granulocyte Macrophage Colony Stimulating Factor; Granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGI1GM; Molgramostin; Pluripoietin-a; Sargramostim

UniProt

P04141

Accession Number

P04141

Expression System

Mammalian

Tag

C-Fc

Sequence

Ala18-Glu144

Field of Research

Cancer; Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

38.9 kDa

Observed Molecular Weight

45-55 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human