Recombinant Human SEMA3A Protein (Fc Tag)

Specifications

Catalog number

PDMH100251-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with an schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with an the progression of Alzheimer's disease.

Abbreviation

SEMA3A

Synonyms

SEMA3A; COLL1; HH16; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SEMAL; SemD; coll-1

UniProt

Q14563

Accession Number

Q14563

Expression System

Mammalian

Tag

C-Fc

Sequence

Lys26-Phe546

Field of Research

Cancer; Cardiovascular; Neuroscience

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

82.2 kDa

Observed Molecular Weight

80-90 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human