Recombinant human CDNF Protein (Fc Tag)

Specifications

Catalog number

PDMH100259-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cerebral Dopamine Neurotrophic Factor (CDNF), also known as ARMETL1 (ARMET-like protein 1), is a secreted protein with eight conserved cysteine residues, predicting a unique protein fold and defining a new, evolutionarily conserved protein family. CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF) . CDNF/ARMETL1 is a evolutionary conserved protein which can protect and restore the function of dopaminergic neurons in the rat model of Parkinson's disease, suggesting that CDNF might be beneficial for the treatment of Parkinson's disease. CDNF is widely expressed in neurons in several brain regions including cerebral cortex, hippocampus, substantia nigra, striatum and cerebellum. Human CDNF is glycosylated and secreted from transiently transfected cells. CDNF promotes the survival, growth, and function of dopamine-specific neurons and is expressed in brain regions that undergo cocaine-induced neuroplasticity.

Abbreviation

CDNF

Synonyms

Arginine rich mutated in early stage tumors like 1; ARMET L1; ARMET like protein 1; ARMET-like protein 1; ARMETL 1; CDNF; CDNF; Cerebral dopamine neurotrophic factor; Conserved dopamine neurotrophic factor

UniProt

Q49AH0

Accession Number

Q49AH0

Expression System

Mammalian

Tag

C-Fc

Sequence

Met1-Leu187

Field of Research

Neuroscience

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.4 kDa

Observed Molecular Weight

40-50 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human