Recombinant Human AXL/UFO Protein (Fc Tag)

Specifications

Catalog number

PDMH100293-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Axl receptor tyrosine kinase , together with Tyro3 and Mer , constitute the TAM family of receptor tyrosine kinases. In the nervous system , Axl and its ligand Growth-arrest-specific protein 6 (Gas6) are expressed on multiple cell types. Axl functions in dampening the immune response , regulating cytokine secretion , clearing apoptotic cells and debris , and maintaining cell survival. Axl is upregulated in various disease states , such as in the cuprizone toxicity-induced model of demyelination and in multiple sclerosis (MS) lesions , suggesting that it plays a role in disease pathogenesis. Axl expression correlates with poor prognosis in several cancers. Axl mediates multiple oncogenic phenotypes and activation of these RTKs constitutes a mechanism of chemoresistance in a variety of solid tumors. Axl contributes to cell survival , migration , invasion , metastasis and chemosensitivity justify further investigation of Axl as novel therapeutic targets in cancer. The receptor tyrosine kinase AXL is thought to play a role in metastasis. The soluble AXL receptor as a therapeutic candidate agent for treatment of metastatic ovarian cancer. GAS6/AXL targeting as an effective strategy for inhibition of metastatic tumor progression in vivo.

Abbreviation

AXL/UFO

Synonyms

JTK; Tyro; AXL; ARK; JTK11; Tyro7; UFO; AXL oncogene; Tyrosine-protein kinase receptor UFO

UniProt

P30530

Accession Number

P30530

Expression System

Mammalian

Tag

C-Fc

Sequence

Ala26–Trp451

Field of Research

Cell Biology; Tags & Cell Markers; Signal Transduction; Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

71.7 kDa

Observed Molecular Weight

90-100 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human