Recombinant Human CEACAM-1/CD66a Protein (Fc Tag)

Specifications

Catalog number

PDMH100298-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1 , also known as biliary glycoprotein I (BGP I) and CD66a , is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD , and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells , epithelial cells , and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners , and was detected on leukocytes , epithelia , and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation , angiogenesis , apoptosis , metastasis , as well as the modulation of innate and adaptive immune responses.

Abbreviation

CEACAM-1/CD66a

Synonyms

Biliary Glycoprotein; Carcinoembryonic Antigen-Related Cell Adhesion Molecule; CEACAM; CEACAM1; BGP; BGP1; BGPI; BGP-1; Biliary Glycoprotein 1; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1; CD66a; NCA-160; Antigen CD66; BGP 1; Biliary glycoprotein adhesion molecule; Carcinoembryonic antigen related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) ; CD66a antigen; CEAM1; meconium antigen 100

UniProt

P13688

Accession Number

P13688

Expression System

Mammalian

Tag

C-Fc

Sequence

Gln35-Gly428

Field of Research

Tags & Cell Markers; Immunology; Cancer; Microbiology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

68.2 kDa

Observed Molecular Weight

90-100 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human