Recombinant Human DR6/TNFRSF21 Protein (His Tag)

Specifications

Catalog number

PDMH100299-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TNFRSF21 (death receptor-6 , DR6) is an orphan TNF receptor superfamily member and belongs to a subgroup of receptors called death receptors. This type I transmembrane receptor possesses four extracellular cysteine-rich motifs and a cytoplasmic death domain. DR6 is an extensively posttranslationally modified transmembrane protein and that N-and O-glycosylations of amino acids in its extracellular part. DR6 interacts with the adaptor protein TRADD and mediates signal transduction through its death domain , and expression of DR6 in mammalian cells induces activation of both NF-kappaB and JNK and cell apoptosis. DR6 knockout mice have enhanced CD4+ T cell proliferation and Th2 cytokine production , suggested that DR6 serves as an important regulatory molecule in T-helper cell activation , and is involved in inflammation and immune regulation. DR6 is expressed ubiquitously with high expression in lymphoid organs , heart , brain and pancreas. Some tumor cells overexpress DR6 , typically in conjunction with elevated anti-apoptosis molecules. DR6 may also be involved in tumor cell survival and immune evasion , which is subject to future investigations.

Abbreviation

DR6/TNFRSF21

Synonyms

UNQ; PRO; Death Receptor; Tumor Necrosis Factor Receptor Superfamily Member; TNFRSF; CD358; Death Receptor 6; Tumor Necrosis Factor Receptor Superfamily Member 21; DR6; TNFRSF21; UNQ437; PRO868; DR6; AA959878 Protein; BM 018; BM018; DR 6; DR6 Protein; MGC31965; OTTHUMP00000039915; R74815 Protein; TNFR related death receptor 6; TNFRSF 21; TNFRSF21 protein; TNR21; TR7 Protein; Tumor necrosis factor receptor superfamily member 21 precursor

UniProt

O75509

Accession Number

O75509

Expression System

Mammalian

Tag

C-His

Sequence

Gln42-His349

Field of Research

Cell Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.8 kDa

Observed Molecular Weight

60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human