Recombinant Human DLL1 Protein (His Tag)

Specifications

Catalog number

PDMH100303-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Delta-like protein 1 (DLL1), also known as Delta1 , a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains , acts as a ligand for Notch receptors , and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor , and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway , whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells , DLL1 is constitutively cleaved by ADAM12 , and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent , takes place in cis orientation , and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1 , but not its N-or C-terminal proteolytic fragment , co-immunoprecipitates with ADAM12. By using a Notch reporter construct , we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore , ADAM9 and ADAM17 have the ability to process DLL1. In contrast , ADAM15 does not cleave DLL1 , although the two proteins still co-immunoprecipitate with each other. During fetal development , DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries.

Abbreviation

DLL1

Synonyms

DLL; UNQ; PRO; Delta-like protein; Drosophila Delta homolog; H-Delta; DLL1; DELTA1; DL1; Delta; Delta-like protein 1; Drosophila Delta homolog 1; H-Delta-1; UNQ146; PRO172

UniProt

O00548

Accession Number

O00548

Expression System

Mammalian

Tag

C-His

Sequence

Ser22-Gly540

Field of Research

Neuroscience; Epigenetics and Nuclear Signaling; Stem Cells; Developmental Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

56.9 kDa

Observed Molecular Weight

70 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human