Recombinant Human SCF/c-kit Ligand Protein (Fc Tag)

Specifications

Catalog number

PDMH100305-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Similar to Kit ligand precursor (C-kit ligand), also known as Stem cell factor (SCF), Mast cell growth factor (MGF), or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development , and hematopoiesis , all believed to reflect a role in cell migration. In adults , it functions pleiotropically , while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein can augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines , probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit , which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro , SCF/C-kit ligand synergizes with other growth factors , such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor , and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid , myeloid , erythroid , and megakaryocytic lineages. In vivo , SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two-to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.

Abbreviation

SCF/c-kit Ligand

Synonyms

FPH; DFNA; SHEP; KITLG; DCUA; DFNA69; FPH2; FPHH; KL-1; Kitl; MGF; SCF; SF; SHEP7; c-Kit ligand; Kit Ligand; Mast Cell Growth Factor; Stem Cell Factor

UniProt

P21583

Accession Number

P21583

Expression System

Mammalian

Tag

C-Fc

Sequence

Glu26-Ala190

Field of Research

Immunology; Stem Cells; Developmental Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.0 kDa

Observed Molecular Weight

50 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human