Recombinant Human BCAM Protein (Fc Tag)

Specifications

Catalog number

PDMH100307-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha (5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region , and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells , Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently , BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166 , and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation , adhesion , migration , and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro , thus being a candidate molecule involved in tumor progression. In addition , BCAM interacts with integrin in sickle red cells , and thus may potentially play a role in vaso-occlusive episodes.

Abbreviation

BCAM

Synonyms

MSK; BCAM; AU; CD239; LU; MSK19; Auberger B antigen; Basal cell adhesion molecule; B-CAM cell surface glycoprotein; F8/G253 antigen; Lutheran antigen; Lutheran blood group glycoprotein; Rh30CE; Antigen identified by monoclonal F8; B CAM cell surface glycoprotein; B cell adhesion molecule; Basal cell adhesion molecule (Lu and Au blood groups) ; Basal cell adhesion molecule (Lutheran blood group) ; Basal cell adhesion molecule Lu and Au blood groups; Basal cell adhesion molecule Lutheran blood group; CD239 antigen; Glycoprotein 95kDa; Lutheran; Lutheran blood group (Auberger b antigen included) ; Lutheran blood group Auberger b antigen included

UniProt

P50895

Accession Number

P50895

Expression System

Mammalian

Tag

C-Fc

Sequence

Glu32-Ala547

Field of Research

Cardiovascular; Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

81.6 kDa

Observed Molecular Weight

90 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human