Recombinant Human Cripto-1 Protein (Fc Tag)

Specifications

Catalog number

PDMH100311-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cripto/TDGF1 is a member of the epidermal growth factor (EGF) -Cripto , Frl-1 , and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif , a conserved cysteine-rich domain , and a C-terminal hydrophobic region. Before gastrulation , Cripto is asymmetrically expressed in a proximal–distal gradient in the epiblast , and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal–distal asymmetry into an orthogonal anterior–posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis , activation of c-Jun-NH (2) -terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus , Cripto is a unique target , and Immunohistochemistry to Cripto could be of therapeutic value for Human cancers.

Abbreviation

Cripto-1

Synonyms

TDGF; Teratocarcinoma-derived growth factor; Epidermal growth factor-like cripto protein CR; TDGF1; CR; CRGF; CRIPTO; Cripto-1 growth factor; Epidermal growth factor-like cripto protein CR1; Teratocarcinoma-derived growth factor 1; Cripto 1; Cripto 1 growth factor; Cripto1 growth factor; Epidermal growth factor like cripto protein CR1; TDGF 1; Teratocarcinoma derived growth factor 1

UniProt

P13385

Accession Number

P13385

Expression System

Mammalian

Tag

C-Fc

Sequence

Leu31-Ser169

Field of Research

Stem Cells; Developmental Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

40.2 kDa

Observed Molecular Weight

40 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human