Recombinant Human EpCAM/TROP-1 Protein (Fc Tag)

Specifications

Catalog number

PDMH100314-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen , is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region , a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage , high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However , recent insights have revealed that EpCAM participates in not only cell adhesion , but also in proliferation , migration and differentiation of cells. In addition , recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain , which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Abbreviation

EpCAM/TROP-1

Synonyms

M4S; MIC; M1S; DIAR; TROP; HNPCC; GA733; TACSTD; EGP; EPCAM; DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1; Ep-CAM; epithelial cell adhesion molecule; GA733-2; M1S2; MK-1; KSA; EGP40; TROP1;17 1A;323/A3;35-KD glycoprotein; Adenocarcinoma associated antigen; Adenocarcinoma-associated antigen; Antigen identified by monoclonal AUA1; AUA1; CD326; CD326 antigen; Cell surface glycoprotein Trop 1; Cell surface glycoprotein Trop 2; Cell surface glycoprotein Trop-1; chromosome 4; CO 17A; CO17 1A; CO17A; EGP 2; EGP2; Ep CAM; EpCAM1; Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) ; Epithelial cell surface antigen; Epithelial cellular adhesion molecule; Epithelial glycoprotein; Epithelial glycoprotein 1; Epithelial glycoprotein 314; EpithelialGlycoprotein; GA733 1; GA733 2; gastrointestinal tumor-associated antigen 2; gp4; hEGP 2; hEGP314; Human epithelial glycoprotein 2; KS 1/4 antigen; KS 1/4 antigen.KSA; Ly74; Lymphocyte antigen 74; M1S 1; Major gastrointestinal tumor associated protein GA733 2; Major gastrointestinal tumor-associated protein GA733-2; mEGP314; Membrane component; Membrane component chromosome 4 surface marker (35kD glycoprotein) ; MK 1; Protein 289A; surface marker; surface marker 1; TACD1; Tumor associated calcium signal transducer 1; Tumor associated calcium signal transducer 2 precursor; Tumor-associated calcium signal transducer 1

UniProt

P16422

Accession Number

P16422

Expression System

Mammalian

Tag

C-Fc

Sequence

Gln24-Lys265

Field of Research

Tags & Cell Markers

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

51.5 kDa

Observed Molecular Weight

60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human