Recombinant Human M-CSF R/CD115 Protein (His Tag)

Specifications

Catalog number

PDMH100319-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation , differentiation , and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain , a single membrane-spanning segment , and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase , leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R , product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors , while tumor cell expression of CSF-1 appears to be regulated by other hormones , some of which are involved in normal lactogenic differentiation. However , studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.

Abbreviation

M-CSF R/CD115

Synonyms

FIM; CSF1R; C-FMS; CD115; CSF-1R; CSFR; FIM2; FMS; HDLS; M-CSF-R; BANDDOS; CSF-1 receptor; CSF-1-R; Macrophage colony-stimulating factor 1 receptor; MCSF Receptor; Proto-oncogene c-Fms; M-CSFR; c-fms; CSF-1R; FMS; C FMS; CD 115; CD115 antigen; CFMS; Colony stimulating factor 1 receptor; Colony stimulating factor I receptor; CSF 1 R; CSF 1R; CSF1 R; EC 2.7.10.1; FIM 2; FMS proto oncogene; FMS protooncogene; formerly; Macrophage colony stimulating factor 1 receptor; Macrophage colony stimulating factor I receptor; McDonough feline sarcoma viral (v fms) oncogene homolog; M-CSF Receptor; MCSF Receptor; MCSFR; Oncogen FMS; V-FMS McDonough feline sarcoma viral oncogen homolog

UniProt

P07333

Accession Number

P07333

Expression System

Mammalian

Tag

C-His

Sequence

Ile20-Glu512

Field of Research

Cardiovascular; Immunology; Signal Transduction

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

54.1 kDa

Observed Molecular Weight

80 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human