Recombinant Human SP-A1 Protein (Fc Tag)

Specifications

Catalog number

PDMH100321-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Surfactant protein A (SFTPA1), a member of the collagenous lectin (collectin) family , was first described as a major constituent of lung surfactant but has recently also been found in the female genital tract. Various microorganisms colonize this area and may cause intrauterine infection or trigger preterm labor. The Human SFTPA1 and SFTPA2 genes encode SP-A1 and SP-2 proteins , and each gene has been identified with numerous genetic variants. SP-A1 and SP-A2 differentially enhance bacterial phagocytosis. Sex differences have been observed in pulmonary disease and survival of wild type and SP-A knockout (KO) mice. SP-A interacts and regulates many of the functions of alveolar macrophages (AM) . It is shown that SP-A variants differ in their ability to regulate the AM miRNome in response to oxidative stress (OxS) .

Abbreviation

SP-A1

Synonyms

MGC; SFTP; Pulmonary surfactant-associated protein A; COLEC; Collectin; SFTPA1; COLEC4; PSAP; PSP-A; PSPA; SFTP1; SFTPA1B; SP-A; SP-A1; SPA; SPA1; MGC133365; Collectin-4; SFTPA; Pulmonary surfactant-associated protein A1;35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein;35 kDa pulmonary surfactant associated protein;35-KD; Collectin 4; FLJ50593; FLJ51913; FLJ61144; FLJ77898; FLJ79095; FLJ99559; MGC198590; OTTHUMP00000019928; OTTHUMP00000019929; OTTHUMP00000019930; OTTHUMP00000019931; PSP A; pulmonary 1; pulmonary associated protein A1A; pulmonary associated protein A1B; pulmonary surfactant apoprotein PSP-A; pulmonary surfactant -associated protein; pulmonary surfactant associated protein A1; SFTA1; SP A; SP A1; surfactant; surfactant protein A1; surfactant protein A1 variant AB'D' 6A2; surfactant protein A1 variant AB'D' 6A3; surfactant protein A1 variant AB'D' 6A4; surfactant protein A1 variant ACD' 6A2; surfactant protein A1 variant ACD' 6A3; surfactant protein A1 variant ACD' 6A4; surfactant protein A1 variant AD' 6A; surfactant protein A1 variant AD' 6A2; surfactant protein A1 variant AD' 6A3; surfactant protein A1 variant AD' 6A4; surfactant protein A1B; surfactant-associated protein

UniProt

Q8IWL2

Accession Number

Q8IWL2

Expression System

Mammalian

Tag

C-Fc

Sequence

Glu21-Phe248

Field of Research

Signal Transduction; Tags & Cell Markers; Cell Biology; Metabolism

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

49.9 kDa

Observed Molecular Weight

60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human