Recombinant Human SPARC Protein (His Tag)

Specifications

Catalog number

PDMH100326-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Secreted protein acidic and rich in cysteine (SPARC), also known as Osteonectin (ON), is a member of the SPARC family. SPARC consists of three domains: an EF-hand domain, a follistatin-like domain and a Kazal-like domain, and each of which has independent activity and unique properties. The activity of SPARC is context-and cell-type-dependent, which is highlighted by the fact that SPARC has shown seemingly contradictory effects on tumor progression in both clinical correlative studies and in animal models. The location of SPARC in the nuclear matrix of certain proliferating cells, but only in the cytosol of postmitotic neurons, indicates potential functions of SPARC as a nuclear protein, which might be involved in the regulation of cell cycle progression and mitosis. It functions not only to modulate cell-cell and cell-matrix interactions, but its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer. Its divergent actions reflect the complexity of this protein, because in certain types of cancers, such as melanomas and gliomas, SPARC is associated with a highly aggressive tumor phenotype, while in others, mainly ovarian, neuroblastomas and colorectal cancers, SPARC may function as a tumor suppressor. Recent studies have also demonstrated a role for SPARC in sensitizing therapy-resistant cancers. Notably, SPARC is linked to human obesity.

Abbreviation

SPARC

Synonyms

Basement-membrane protein; SPARC; BM-40; OI17; Osteonectin; acidic; cysteine-rich; Basement-membrane protein 40; Oncysteine-rich protein; Secreted protein acidic and rich in cysteine; secreted protein; ON; AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and cysteine rich; Secreted protein acidic cysteine rich; Secreted protein acidic cysteine rich (osteonectin) ; SPRC

UniProt

P09486

Accession Number

P09486

Expression System

Mammalian

Tag

C-His

Sequence

Met1-lle303

Field of Research

Cell Biology; Cardiovascular; Signal Transduction; Stem Cells; Cancer; Developmental Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.2 kDa

Observed Molecular Weight

38 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human