Recombinant Human Try-2 Protein (His Tag)

Specifications

Catalog number

PDMH100327-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Trypsin-2, also known as Trypsin II, Anionic trypsinogen, Serine protease 2, PRSS2 and TRY2, is a secreted protein that belongs to the trypsin serine protease family including Trypsin, PRSS1, PRSS2 and PRSS3. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a proteolytically active mature chain (residues 24-247) . PRSS2 contains one peptidase S1 domain. It is secreted into the duodenum, hydrolysing peptides into their smaller building blocks, which is necessary for the uptake of protein in the food. It is secreted by the pancreas in the form of inactive zymogen, trypsinogen and cleaved to its active form in the small intestine when the pancreas is stimulated by cholecystokinin throμgh the common activation mechanism.

Abbreviation

Try-2

Synonyms

PRSS; TRYP; Tesp; Serine protease; TRY2TRY; EC 3.4.; TRY; PRSS2; TRY2; TRY8; TRYP2; trypsin-2; serine; preproprotein; protease; MGC111183; Tesp4; MGC120174; TRY2TRY8; MGC120175; Anionic trypsinogen;2 (trypsin 2) ; EC 3.4.21; EC 3.4.21.4; Serine protease 2; protease serine 2 preproprotein; Trypsin 2; Trypsin II; trypsinogen 2; Trypsinogen 20

UniProt

P07478

Accession Number

P07478

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Ser247

Field of Research

Signal Transduction; Metabolism; Cell Biology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.1 kDa

Observed Molecular Weight

30 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human