Recombinant human Flt-3 Ligand Protein (His Tag)

Specifications

Catalog number

PDMH100328-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.

Abbreviation

Flt-3 Ligand

Synonyms

FL; Flt3 Ligand; Flt3-L; Fms-Felated Tyrosine Kinase 3 Ligand; Fms-Related Tyrosine Kinase 3 ligand; SL Cytokine; FLT3LG; Flt3L

UniProt

P49771

Accession Number

P49771

Expression System

Mammalian

Tag

C-His

Sequence

Thr27-Pro184

Field of Research

Cardiovascular; Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

17.3 kDa

Observed Molecular Weight

25-35 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human