Recombinant Human Decorin Protein (His Tag)

Specifications

Catalog number

PDMH100329-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS) . As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. Decorin resides in the tumor microenvironment and affects the biology of various types of cancer by downregulating the activity of several receptors involved in cell growth and survival. Decorin binds to and modulates the signaling of the epidermal growth factor receptor and other members of the ErbB family of receptor tyrosine kinases. It exerts its antitumor activity by a dual mechanism: via inhibition of these key receptors throμgh their physical downregulation coupled with attenuation of their signaling, and by binding to and sequestering TGFbeta. Decorin also modulates the insulin-like growth factor receptor and the low-density lipoprotein receptor-related protein 1, which indirectly affects the TGFbeta receptor pathway. Decorin plays significant roles in tissue development and assembly, as well as playing both direct and indirect signaling roles.

Abbreviation

Decorin

Synonyms

PG-S; PG S; DKFZp686J; PGS; DCN; CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B; decorin; DCN protein; Decorin proteoglycan; Dermatan sulphate proteoglycans II; DKFZp686J19238; DSPG 2; PG 40; PG II; PG S2; PGS 2; Proteoglycan core protein; Small leucine rich protein 1B; PG-S2; Bone proteoglycan II

UniProt

P07585

Accession Number

P07585

Expression System

Mammalian

Tag

C-His

Sequence

Asp31-Lys359

Field of Research

Signal Transduction; Stem Cells

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.1 kDa

Observed Molecular Weight

45 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human