Recombinant Human CD30 Ligand/TNFSF8 Protein (His Tag)

Specifications

Catalog number

PDMH100341-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD30 ligand (CD30L), also known as CD153 and TNFSF8, is a membrane-associated glycoprotein belonging to the TNF superfamily and TNFR superfamily, and is a specific ligand for CD30/TNFRSF8 originally described as a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. CD30L is a type-II membrane glycoprotein expressed on activated T cells, stimulated monocyte-macrophages, granulocytes, eosinophils, and some Burkitt-like lymphoma cell lines. CD30L is capable of transducing signals throμgh CD30 on different CD30+ lymphoma cell lines, and mediates pleiotropic biologic effects including cell proliferation, activation, differentiation, as well as cell death by apoptosis. CD30-CD30 ligand interaction has been sμggested to have a pathophysiologic role in malignant lymphomas, particularly Hodgkin disease, large cell anaplastic lymphomas and Burkitt lymphomas, and is also involved in activation and functioning of the T cell-dependent immune response. Thus, CD153 and its receptor CD30 are regarded as therapeutic targets in hematologic malignancies, autoimmune and inflammatory diseases.

Abbreviation

CD30 Ligand/TNFSF8

Synonyms

PSOR; CD antigen CD; Tumor necrosis factor ligand superfamily member; TNFSF; S100A; TNFSF8; CD153; CD30L; CD30LG; TNLG3A; CD antigen CD153; CD30 ligand; CD30-L; sCD30 Ligand; soluble CD30 Ligand; Tumor necrosis factor ligand superfamily member 8; S100A7; PSOR1; S100A7C; TNFSF8

UniProt

P32971

Accession Number

P32971

Expression System

Mammalian

Tag

C-His

Sequence

Val63-Asp234

Field of Research

Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.8 kDa

Observed Molecular Weight

40 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human