Recombinant Human Fibroblast Activation Protein α/FAP Protein (His Tag)

Specifications

Catalog number

PDMH100344-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Seprase, also known as 17 KD melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to thepeptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 17-KD dimer, consisting of two 97-KD subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV (DPPIV / CD26) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-associated molecules. In association with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

Abbreviation

Fibroblast Activation Protein α/FAP

Synonyms

170 kDa melanoma membrane bound gelatinase;170 kDa melanoma membrane-bound gelatinase; DPPIV; DPPIVA; FAPA; Fibroblast activation protein alpha; Integral membrane serine protease; SEPR; Seprase; SIMP; FAP; FAPalpha

UniProt

Q12884

Accession Number

Q12884

Expression System

Mammalian

Tag

C-His

Sequence

Leu26-Asp760

Field of Research

Cell Biology; Signal Transduction; Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

80.7 kDa

Observed Molecular Weight

90 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human