Recombinant Human PLAUR Protein (His Tag)

Specifications

Catalog number

PDMH100351-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Urokinase plasminogen activator (uPA) and/or its receptor (uPAR) are essential for metastasis, and overexpression of these molecules is strongly correlated with poor prognosis in a variety of malignant tumours. uPAR and uPA levels in both resected tumor tissue and plasma are of independent prognostic significance for patient survival in several types of human cancer. This system has classically been thoμght to drive tumor progression by mediating directed extracellular proteolysis on the surface of migrating or invading cells, and intervening with this proteolysis by targeting uPAR has been proposed to represent a novel approach for inhibiting tumor progression. uPAR, also known as PLAUR or CD87, has been implicated in the growth, metastasis, and angiogenesis of several solid and hemotologic malignancies. uPAR is a highly glycosylated, 55-60 KD integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. It is part of a cell surface system that also consists of the serine protease uPA and several specific inhibitors (plasminogen activator inhibitors 1 and 2) . Additionally, the analysis of CD87 (urokinase-type plasminogen activator receptor-uPAR) expression has a potential role in the diagnostic or prognostic work-up of several hematological malignancies, particularly acute leukemia and multiple myeloma.

Abbreviation

PLAUR

Synonyms

PLAUR; CD87; U-PAR; UPAR; URKR; Monocyte activation antigen; suPAR; Urokinase Plasminogen Activator Surface Receptor; MO3; CD 87; CD87 antigen; MO 3; Monocyte activation antigen Mo3; Plasminogen activator receptor urokinase; Plasminogen activator urokinase receptor; U PAR; u plasminogen activator receptor; UPA receptor; u-plasminogen activator receptor form 2; Urinary plasminogen activator receptor; Urokinase plasminogen activator receptor; urokinase-type plasminogen activator (uPA) receptor

UniProt

Q03405

Accession Number

Q03405

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Arg303

Field of Research

Cardiovascular; Immunology; Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.2 kDa

Observed Molecular Weight

45 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human