Recombinant Mouse Prostatic Acid Phosphatase Protein (Fc Tag)

Specifications

Catalog number

PDMM100101-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ACPP (Acid phosphatase, prostate, also PAP and ACP3) is a 48-52 kDa glycoprotein member of the histidine acid phosphatase family of enzymes. It exists as a 95-100 kDa nondisulfide-linked homodimer that hydrolyzes phosphate esters under low pH to generate free phosphate. ACPP is expressed by prostate epithelium and pain-detecting spinal cord neurons. In the spinal cord, ACPP dephosphorylates AMP. This generates adenosine which acts as a strong analgesic agent. Mature Human ACPP is 354 amino acids (aa) in length (aa 33-386) . It contains one histidine phosphatase domain (aa 34-332), plus a nucleophile acceptor site at His44, and a proton donor site at Asp290. There are two potential alternative splice variants. One shows a deletion of aa 153-185, while another is transmembrane (previously called TMPase) and shows a 38 aa substitution for the C-terminal seven amino acids. Over aa 33-379, Human ACPP shares 84% aa identity with an Mouse ACPP.

Abbreviation

Prostatic Acid Phosphatase/ACPP

Synonyms

5'-NT;5'-nucleotidase; Acid phosphatase prostate; ACP 3; ACP3; acpP; Ecto-5'-nucleotidase; PAP; PPAP; Prostatic acid phosphatase; Prostatic acid phosphotase; Thiamine monophosphatase; TMPase

UniProt

Q8CE08

Accession Number

Q8CE08

Expression System

Mammalian

Tag

C-Fc

Sequence

Met1-Asn381aa

Field of Research

Tags & Cell Markers; Cell Biology; Cancer

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

66.8 kDa

Observed Molecular Weight

80 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse