Recombinant Mouse CD30 Ligand/TNFSF8 Protein (Fc Tag)

Specifications

Catalog number

PDMM100135-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD30 ligand (CD30L), also known as CD153 and TNFSF8 , is a membrane-associated glycoprotein belonging to the TNF superfamily and TNFR superfamily , and is a specific ligand for CD30/TNFRSF8 originally described as a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. CD30L is a type-II membrane glycoprotein expressed on activated T cells , stimulated monocyte-macrophages , granulocytes , eosinophils , and some Burkitt-like lymphoma cell lines. CD30L is capable of transducing signals through CD30 on different CD30+ lymphoma cell lines , and mediates pleiotropic biologic effects including cell proliferation , activation , differentiation , as well as cell death by apoptosis. CD30-CD30 ligand interaction has been suggested to have a pathophysiologic role in malignant lymphomas , particularly Hodgkin disease , large cell anaplastic lymphomas and Burkitt lymphomas , and is also involved in activation and functioning of the T cell-dependent immune response. Thus , CD153 and its receptor CD30 are regarded as therapeutic targets in hematologic malignancies , autoimmune and inflammatory diseases.

Abbreviation

CD30 Ligand/TNFSF8

Synonyms

TNFSF; Cd30l; Cd30lg; Tnfsf8; TNFSF8; CD153; CD30 ligand; Tumor necrosis factor ligand superfamily member 8

UniProt

P32972

Accession Number

P32972

Expression System

Mammalian

Tag

C-Fc

Sequence

Gln68-Asp239

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.8 kDa

Observed Molecular Weight

55-65 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse