Recombinant Mouse BCAM Protein (Fc Tag)

Specifications

Catalog number

PDMM100159-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha (5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region , and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells , Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently , BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166 , and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation , adhesion , migration , and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro , thus being a candidate molecule involved in tumor progression. In addition , BCAM interacts with integrin in sickle red cells , and thus may potentially play a role in vaso-occlusive episodes.

Abbreviation

BCAM

Synonyms

3732419F; C230027C;1200005K12Rik;2900075O15Rik;3732419F12; AI844366; B930023M13Rik; B-CAM; C230027C17; Gm181; Obcam; Gplu; Lu; Bcam

UniProt

Q9R069

Accession Number

Q9R069

Expression System

Mammalian

Tag

C-Fc

Sequence

Glu26-Ala541

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

81.7 kDa

Observed Molecular Weight

90 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse