Recombinant Mouse E-Cadherin Protein (His Tag)

Specifications

Catalog number

PDMM100161-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells , and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324 , is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer , lobular breast cancer , and endometrial cancer. In Human cancers , partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx , E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor , as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system , and often correlates with strong invasive potential and poor prognosis of Human carcinomas.

Abbreviation

E-Cadherin

Synonyms

CDH; CAM 120/; AA960649; ARC-1; CAM 120/80; CD324; CDHE; Ecad; E-cad; Epithelial Cadherin; LCAM; L-CAM; Um; UVO; Uvomorulin; Cdh1; E-Cadherin; CDH1

UniProt

P09803

Accession Number

P09803

Expression System

Mammalian

Tag

C-His

Sequence

Asp157-Val709

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

60.7 kDa

Observed Molecular Weight

80 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse