Recombinant Mouse VCAM-1/CD106 Protein (Fc Tag)

Specifications

Catalog number

PDMM100163-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106 , is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing , with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes , and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease , atherosclerosis , allograft rejection , infection , and asthmatic responses. During these responses , VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target , the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition , VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.

Abbreviation

VCAM-1/CD106

Synonyms

MGC; Vascular cell adhesion protein; Vascular Cell Adhesion Molecule; VCMA; V-CAM; CD106; CD106 Antigen; INCAM 100; INCAM-100; L1CAM; MGC99561; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; V-CAM 1; VCMA1; Vcam-1; Vcam1; CD106; INCAM-100; vascular cell adhesion molecule 1

UniProt

P29533

Accession Number

P29533

Expression System

Mammalian

Tag

C-Fc

Sequence

Met1-Glu698

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

101.6 kDa

Observed Molecular Weight

102 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse