Recombinant Mouse ICAM-1/CD54 Protein (Fc Tag)

Specifications

Catalog number

PDMM100165-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Intercellular adhesion molecule-1 (ICAM-1 , or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells , but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally , ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1 (integrin) . When activated , leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics , ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of Human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.

Abbreviation

ICAM-1/CD54

Synonyms

MyD; P3.; MALA; ICAM-1/CD; MAL; CD54; Icam; Ly-4; Ly-47; Icam-1; MALA-2; BB2; ICAM-1/CD54; Intercellular adhesion molecule 1; MyD10; P3.58; Icam1; Ly-47; MALA-2; CD54; Icam; Icam-1; Ly-4

UniProt

P13597

Accession Number

P13597

Expression System

Mammalian

Tag

C-Fc

Sequence

Gln28-Asn485

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

75.2 kDa

Observed Molecular Weight

90 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse