Recombinant Mouse CD40/TNFRSF5 Protein (His Tag)

Specifications

Catalog number

PDMM100170-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3) . In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thoμght to be an early event in Alzheimer disease pathogenesis.

Abbreviation

CD40/TNFRSF5

Synonyms

IMD; B-cell surface antigen CD; Tumor necrosis factor receptor superfamily member; HIGM; Tnfrsf; HIGM1; I326936; IGM; IMD3; p50; T-BAM; TRAP; Bp50; CD40; Tnfrsf5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; CD40L receptor; gp39 R; TNFRSF5; CD40 (protein) ; CD40 molecule; CDW40

UniProt

P27512

Accession Number

P27512

Expression System

Mammalian

Tag

C-His

Sequence

Leu20-Arg193

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19 kDa

Observed Molecular Weight

30 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse