Recombinant Mouse EpCAM/TROP-1 Protein (Fc Tag)

Specifications

Catalog number

PDMM100174-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.

Abbreviation

EpCAM/TROP-1

Synonyms

EGP; Megp; Tacstd; EGP314; Ep-CAM; Megp314; Tacstd1; Epcam; MK-1; KSA; EGP40;17 1A;323/A3;35-KD glycoprotein; Adenocarcinoma associated antigen; Adenocarcinoma-associated antigen; Antigen identified by monoclonal AUA1; AUA1; CD326; CD326 antigen; Cell surface glycoprotein Trop 1; Cell surface glycoprotein Trop 2; Cell surface glycoprotein Trop-1; chromosome 4; CO 17A; CO17 1A; CO17A; DIAR5; EGP 2; EGP2; EGP-2; EGP40; Ep CAM; EpCAM1; epithelial cell adhesion molecule; Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) ; Epithelial cell surface antigen; Epithelial cellular adhesion molecule; Epithelial glycoprotein; Epithelial glycoprotein 1; Epithelial glycoprotein 314; ESA; GA733 1; GA733 2; GA733-2; gastrointestinal tumor-associated antigen 2; gp4; gp40; hEGP 2; hEGP314; HNPCC8; Human epithelial glycoprotein 2; KS 1/4 antigen; KS1/4; KSA; Ly74; Lymphocyte antigen 74; M1S 1; M1S2; M4S1; Major gastrointestinal tumor associated protein GA733 2; Major gastrointestinal tumor-associated protein GA733-2; Membrane component; Membrane component chromosome 4 surface marker (35kD glycoprotein) ; MIC18; MK 1; MK-1; Neurotrophic tyrosine kinase receptor-related 1; Protein 289A; receptor tyrosine kinase-like orphan receptor 1; ROR1; surface marker; surface marker 1; TACD1; Tacsd1; TROP1; Tumor associated calcium signal transducer 1; Tumor associated calcium signal transducer 2 precursor; Tumor-associated calcium signal transducer 1; tyrosine-protein kinase transmembrane receptor ROR1

UniProt

Q99JW5

Accession Number

Q99JW5

Expression System

Mammalian

Tag

C-Fc

Sequence

Gln24-Lys266

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

51.6 kDa

Observed Molecular Weight

55 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Mouse