Recombinant Human CDK2 Protein (E.coli, His Tag)

Specifications

Catalog number

PDEH100671-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cyclin-dependent kinase 2 (CDK2) belongs to the cyclin-dependent kinase of Ser/Thr protein kinase. CDK2 acts as a catalytic subunit of the cyclin dependent kinase complex, whose activity is restricted to the G1-S phage of the cell cycle, it is essential for the G1/S transition. The kinase activity of CDK2 can be regulated by the association with a cyclin subunit, its phosphorylation state and CDK inhibitors. The activation of the CDK2/cyclin complex requires the phosphorylation of Thr160 and the dephosphorylation of Try14 and Tyr15. The inhibition of CDK2-cyclin complex can also be attributed to association with p27Kip1 and p21Waf1/Cip1. The activation of CDK2 has been shown to be necessary for apoptosis of quiescent cells, such as neurons, thymocytes and endothelial cells.

Abbreviation

CDK2

Synonyms

CDK2; CDKN2; Cell Division Protein Kinase 2; Cyclin-Dependent Kinase 2; p33 Protein Kinase

UniProt

P24941

Accession Number

P24941

Expression System

E.coli

Tag

N-His

Sequence

Met 1-Leu298

Field of Research

Signal Transduction; Cell biology; Neuroscience; Cancer; epigenetics and nuclear signal; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.1 kDa

Observed Molecular Weight

34 kDa

Species

Human