Recombinant Human PBEF/NAMPT Protein (His Tag)

Specifications

Catalog number

PDEH100673-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “visfatin”. PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF-α, IL-1β, IL-16, and TGF-β1. PBEF also increases the production of IL-6, TNF-α, and IL-1β in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells.

Abbreviation

PBEF; NAMPT

Synonyms

NAMPT; NAmPRTase; Nampt; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre-B cell-enhancing factor; Pre-B-cell colony-enhancing factor 1; Visfatin

UniProt

P43490

Accession Number

P43490

Expression System

E.coli

Tag

N-His

Sequence

Met1-His491

Field of Research

Signal Transduction; Cardiovascular; Neuroscience; Cancer; immunology; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.0 kDa

Observed Molecular Weight

55 kDa

Species

Human