Recombinant Human SRC/Proto-oncogene c-Src Protein (His Tag)

Specifications

Catalog number

PDEH100688-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is constitutively active as opposed to normal SRC (C-SRC) . Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

Abbreviation

SRC; Proto-oncogene c-Src

Synonyms

Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; SRC1; p60-Src; pp60c-src

UniProt

P12931

Accession Number

P12931-1

Expression System

E.coli

Tag

N-His

Sequence

Gly2-Gly287

Field of Research

Signal Transduction, Epigenetics and Nuclear Signaling, Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

31.2 kDa

Observed Molecular Weight

32.4 kDa

Species

Human