Recombinant Human NGAL/LCN2 Protein (His Tag)

Specifications

Catalog number

PDEH100692-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. It was initially found in activated neutrophils, however, many other cells, like kidney tubular cells, may produce NGAL in response to various insults. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI) . Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein which specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis.

Abbreviation

NGAL; LCN2

Synonyms

24p3;25 kDa Alpha 2 Microglobulin Related Subunit of MMP9; Alpha 2 Microglobulin Related Protein; HGNC:6526; Lcn 2; Lipocalin-2; MSFI; Migration Stimulating Factor Inhibitor; Neu; Oncogene 24p3; SV40 induced 24P3 Protein; Siderocalin; Siderocalin LCN2; Uterocalin; p25

UniProt

P80188

Accession Number

P80188-1

Expression System

E.coli

Tag

N-His

Sequence

Gln21-Gly198

Field of Research

Immunology, Cardiovascular, Signal Transduction, Cancer, Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.5 kDa

Observed Molecular Weight

19 kDa

Species

Human