Recombinant Human Merlin/NF2 protein (His Tag)

Specifications

Catalog number

PDEH100698-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

Abbreviation

Merlin; NF2

Synonyms

BANF; Bilateral acoustic neuroma; CAN; MERL_HUMAN; Merlin; Moesin ezrin radixin like protein; Moesin ezrin radizin like; Moesin-ezrin-radixin-like; NF 2; Neurofibromatosis 2; Neurofibromatosis type 2; Neurofibromatosis2; SCH; Schwannomerlin

UniProt

P35240

Accession Number

P35240-1

Expression System

E.coli

Tag

N-His

Sequence

Met1-Leu595

Field of Research

Neuroscience, Signal Transduction, Epigenetics and Nuclear Signaling, Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

69.7 kDa

Observed Molecular Weight

68-71 kDa

Species

Human