Recombinant Human GAPDH protein (His Tag)

Specifications

Catalog number

PDEH100699-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity) . Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Abbreviation

GAPDH

Synonyms

Aging associated gene 9 protein; Epididymis secretory sperm binding protein Li 162eP; G3P; G3PD; G3PDH; GAPD; GAPDH; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; HEL S 162eP; Peptidyl-cysteine S-nitrosylase GAPDH

UniProt

P04406

Accession Number

P04406

Expression System

E.coli

Tag

N-His

Sequence

Met1-Glu333

Applications

Immunogen

Field of Research

Signal Transduction; Cancer; Isotypeloading control; metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.1 kDa

Observed Molecular Weight

34-37 kDa

Species

Human