Recombinant Human BRCA2 Protein (His Tag)

Specifications

Catalog number

PDEH100705-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

Abbreviation

BRCA2

Synonyms

BRCA 2; BRCA1/BRCA2 containing complex subunit 2; BRCA2; BRCC 2; BRCC2; Brca2; Breast Cancer 2 t; Breast and ovarian cancer susceptibility gene early onset; Breast cancer 2 early onset; DNA repair associated; breast and ovarian cancer susceptibility protein 2

UniProt

P51587

Accession Number

P51587-1

Expression System

E.coli

Tag

N-His

Sequence

Thr2350-Val2545

Applications

Immunogen

Field of Research

Epigenetics and Nuclear Signaling, Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.1 kDa

Observed Molecular Weight

25.4 kDa

Species

Human