Recombinant Human CDH1 Protein (His Tag)

Specifications

Catalog number

PDEH100708-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.

Abbreviation

CDH1

Synonyms

Arc 1; CADH1; CAM 120/80; CD 324; CD324; CD324 antigen; CDHE; Cadherin 1; Cadherin1; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin; cadherin 1 type 1 E-cadherin; cdh1; epithelial calcium dependant adhesion protein

UniProt

P12830

Accession Number

P12830-1

Expression System

E.coli

Tag

N-His

Sequence

Asp155-Phe375

Applications

Immunogen

Field of Research

Signal Transduction, Cancer, Developmental Biology, Tags & Cell Markers

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.9 kDa

Observed Molecular Weight

35 kDa

Species

Human