Recombinant Human CDK4 Protein (His Tag)

Specifications

Catalog number

PDEH100709-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a) . This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb) . Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Abbreviation

CDK4

Synonyms

CDK4; CDK4 protein; CMM 3; CMM3; Cdk 4; Cell division kinase 4; Cell division protein kinase 4; Crk3; Cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; Melanoma cutaneous malignant 3; PSK J3; PSK-J3; cdk4; p34 cdk4

UniProt

P11802

Accession Number

P11802-1

Expression System

E.coli

Tag

N-His

Sequence

Ala2-Glu303

Field of Research

Cell Biology, Epigenetics and Nuclear Signaling, Cancer, Isotype, Loading Controls

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.5 kDa

Observed Molecular Weight

35.8 kDa

Species

Human