Recombinant Human EGFR Protein (His Tag)

Specifications

Catalog number

PDEH100710-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.

Abbreviation

EGFR

Synonyms

Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epide; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog)

UniProt

P00533

Accession Number

P00533-1

Expression System

E.coli

Tag

N-His

Sequence

Glu204-His370

Field of Research

Signal Transduction, Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

18.2 kDa

Observed Molecular Weight

20.5 kDa

Species

Human