Recombinant Human CXCR4 (C28A) Protein (His Tag)

Specifications

Catalog number

PDEH100716-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin, leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.

Abbreviation

CXCR4

Synonyms

C-X-C chemokine receptor type 4; CD184; FB22; Fusin; HM89; HSY3RR; LAP3; LCR1; NPYR; SDF 1 receptor; WHIM

UniProt

P61073

Accession Number

P61073

Expression System

E.coli

Tag

N-GST

Sequence

Met1-Lys38

Field of Research

Microbiology; Neuroscience; Cancer; immunology; metabolism; Stem cells

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

31.2 kDa

Observed Molecular Weight

32-35 kDa

Species

Human