Recombinant Human KI67 protein (His tag)

Specifications

Catalog number

PDEH100761-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly.Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface.Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility.Binds DNA, with a preference for supercoiled DNA and AT-rich DNA.Does not contribute to the internal structure of mitotic chromosomes.May play a role in chromatin organization.It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable) .

Abbreviation

KI67

Synonyms

Antigen KI-67; Antigen Ki67; Antigen identified by monoclonal antibody Ki-67

UniProt

P46013

Accession Number

P46013

Expression System

E.coli

Tag

N-His

Sequence

Lys1001-Pro1112

Field of Research

Cell Biology, Neuroscience, Tags & Cell Markers, Cancer

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

12.4 kDa

Observed Molecular Weight

13 kDa

Species

Human