Recombinant Human PPARG protein (His tag)

Specifications

Catalog number

PDEH100762-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels.

Abbreviation

PPARG

Synonyms

Nuclear receptor subfamily 1 group C member 3; PPAR-gamma; PPAR-γ

UniProt

P37231

Accession Number

P37231

Expression System

E.coli

Tag

N-His

Sequence

Pro234-Tyr505

Field of Research

Epigenetics and Nuclear Signaling, Neuroscience, Stem Cells, Cardiovascular, Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

28.0 kDa

Observed Molecular Weight

20 kDa

Species

Human