Recombinant Human Orexin-A protein (TRX, His tag)

Specifications

Catalog number

PDEH100781-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested.

Abbreviation

Orexin-A

Synonyms

HCRT; Hcrt1; Hypocretin-1; OX; Orexin-A; PPORX; PPOX

UniProt

O43612

Accession Number

O43612

Expression System

E.coli

Tag

N-Trx-His

Sequence

Gln34-Leu66

Field of Research

Neuroscience, Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.5 kDa

Observed Molecular Weight

25 kDa

Species

Human