Recombinant Human Collagen alpha-1 (I) chain/COL1A1 protein (TRX, His tag)

Specifications

Catalog number

PDEH100790-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Abbreviation

Collagen alpha-1 chain; COL1A1

Synonyms

Alpha-1 type I collagen; COL1A1; Collagen alpha-1 (I) chain

UniProt

P02452

Accession Number

P02452

Expression System

E.coli

Tag

N-Trx-His

Sequence

Glu 1021-Gly 1109

Field of Research

Signal Transduction; Cell biology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

29.7 kDa

Observed Molecular Weight

32 kDa

Species

Human