Recombinant Human AMPK1 protein (GST tag)

Specifications

Catalog number

PDEH100794-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms (alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Abbreviation

AMPK1

Synonyms

ACACA kinase; AMPK subunit alpha-1; AMPK1; PRKAA1

UniProt

Q13131

Accession Number

Q13131-2

Expression System

E.coli

Tag

N-GST

Sequence

Lys 421-Gln 574

Field of Research

Neuroscience; Signal Transduction; Cardiovascular; Cancer; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

41.8 kDa

Observed Molecular Weight

45 kDa

Species

Human